Polani B. Seshagiri  

   
                   
  Indian Institute of Science  

Embryo Biology

 

  Dept of MRDG    
   
  Lab Members   Sperm Biology Embryo biology Stem Cell Biology  
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      Following successful fertilization, preimplantation embryos undergo sequential cleavage divisions and form blastocysts. They then escape from the glycoproteinaceous coat viz., zona pellucida, by a phenomenon known as ‘hatching’. We are studying the cellular and molecular regulation of this critical peri-implantation developmental phenomenon and the bi-directional signaling mechanisms involving embryo-endometrial tissues, which engage a complex and a coordinated interplay of several growth factors (EGF, HB-EGF, LIF), transcription factors (Cdx-2, Oct-4), and proteases (cathepsins). The effects of these and other molecules during early development, especially during the period of blastocyst hatching are being investigated. We also employ the non-human primate (Macaca radiata) model to understand blastocyst development and differentiation and their regulation by endocrine factors. In order to obtain multiple primate embryos, we attempt to induce multiple ovulations and generation of multiple embryos using human gonadotrophin-induced ovarian hyperstimulation approach.

 
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Working hypothesis on the cellular and molecular regulation of blastocyst hatching

Hamster 8-cell embryo development in vitro