Polani B. Seshagiri

 
             
  Indian Institute of Science  

Stem Cell and Transgenic Research Lab

 
Dept of MRDG  
  Lab Members   Sperm Biology Embryo biology Stem Cell Biology    
  Collaborators  

      Embryonic Stem cells (ES-cell) are pluripotent cells derived from the inner cell mass of blastocyst. Though, the human ES-cell lines have been successfully derived, their clinical use for potential cell-based therapy is far from being tested in view of the lack of our understanding of cell lineage specific differentiation, homing mechanisms and functional integration into the host tissue.  We intend to use wild-type and enhanced green fluorescent protein (EGFP)-expressing mouse ES-cells to understand cell lineage-specific differentiation and for experimental cell transplantation studies. Mouse ES-cells, being generated from EGFP expressing transgenic ‘green’ mice (generated in our laboratory), are used. We are studying the role of Wnt and FGF signaling pathways during ES-cell differentiation into functional cardiomyocytes. Using EGFP-transgenic “green” mice, we are also studying the transgene expression pattern during germ cell development and embryogenesis. 

 
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  Development of transgenic blastocyst  

EGFP expression during mouse development

 
     
     
     
     
     
     
       
 

Blastocyst

   
     
     
     
     
       
 

Hatched blastocyst

   
     
     
     
     
     
         
 

Attached blastocyst

 

     Techniques employed for the study include primary cultures of mammalian gametes, embryos and ES-cells, micromanipulation, molecular biology and immunological methods, immunofluorescence microscopy, morphometric analysis and real time videomicrography during early embryonic development and differentiation.

 
     
     
             
   

Stem Cell Lab

 
     
       
 

ES-cells

   
     
     
     
     
     
     
       
 

Embryoid Body