Home Research Activities Lab Members Publications Collaborators 



Singhal A, Arora G, Virmani R, Kundu P, Khanna T, Sajid A, Misra R, Joshi J, Yadav V, Samanta S, Saini N, Pandey AK, Visweswariah SS, Hentschker C, Becher D, Gerth U, Singh Y.

Systematic analysis of mycobacterial acylation reveals first example of acylation-mediated regulation of enzyme activity of a bacterial phosphatase.


Visweswariah SS, Busby SJ.

Evolution of bacterial transcription factors: how proteins take on new tasks, but do not always stop doing the old ones.


Müller T, Rasool I, Heinz-Erian P, Mildenberger E, Hülstrunk C, Müller A, Michaud L, Koot BG, Ballauff A, Vodopiutz J, Rosipal S, Petersen BS, Franke A, Fuchs I, Witt H, Zoller H, Janecke AR, Visweswariah SS.

Congenital secretory diarrhoea caused by activating germline mutations in GUCY2C.

Banerjee A, Adolph RS, Gopalakrishnapai J, Kleinboelting S, Emmerich C, Steegborn C, Visweswariah SS.

A Universal Stress Protein (USP) in Mycobacteria Binds cAMP.


Biswas KH, Badireddy S, Rajendran A, Anand GS, Visweswariah

Cyclic nucleotide binding and structural changes in the isolated GAF domain of Anabaena adenylyl cyclase, CyaB2.


Barathy DV, Bharambe NG, Syed W, Zaveri A, Visweswariah SS, Colaco M, Misquith S, Suguna K.

Autoinhibitory mechanism and activity-related structural changes in a mycobacterial adenylyl cyclase.


Matange N, Podobnik M, Visweswariah SS.

Metallophosphoesterases: structural fidelity with functional promiscuity.

Biochem J. 2015 Apr 15;467(2):201-16.


Wang L, Zhang X, Wang G, Visweswariah SS, Lin G, Xin Z, Lue TF, Lin CS.

Lobe-specific expression of phosphodiesterase 5 in rat prostate.


Kumar S, Matange N, Umapathy S, Visweswariah SS.

Linking carbon metabolism to carotenoid production in mycobacteria using Raman spectroscopy.


Sharma R, Zaveri A, Gopalakrishnapai J, Srinath T, Varshney U, Visweswariah SS.

Paralogous cAMP receptor proteins in Mycobacterium smegmatis show biochemical and functional divergence.


Sharma S, Zaveri A, Visweswariah SS, Krishnan Y.

A fluorescent nucleic acid nanodevice quantitatively images elevated cyclic adenosine monophosphate in membrane-bound compartments.


Pedroza-Roldán C, Aceves-Sánchez Mde J, Zaveri A, Charles-Nińo C, Elizondo-Quiroga DE, Hernández-Gutiérrez R, Allen K, Visweswariah SS, Flores-Valdez MA.

The adenylyl cyclase Rv2212 modifies the proteome and infectivity of Mycobacterium bovis BCG.


Matange N, Podobnik M, Visweswariah SS.

The non-catalytic "cap domain" of a mycobacterial metallophosphoesterase regulates its expression and localization in the cell.


Kahramanoglou C, Cortes T, Matange N, Hunt DM, Visweswariah SS, Young DB, Buxton RS.

Genomic mapping of cAMP receptor protein (CRP Mt) in Mycobacterium tuberculosis: relation to transcriptional start sites and the role of CRPMt as a transcription factor.

Nucleic Acids Res. 2014 Jul;42(13):8320-9.


Podobnik M, Siddiqui N, Rebolj K, Nambi S, Merzel F, Visweswariah SS.

Allostery and conformational dynamics in cAMP-binding acyltransferases.

J Biol Chem. 2014 Jun 6;289(23):16588-600.


Basu N, Saha S, Khan I, Ramachandra SG, Visweswariah SS.

Intestinal Cell Proliferation and Senescence Are Regulated by Receptor Guanylyl Cyclase C and p21.

J Biol Chem. 2014 Jan 3;289(1):581-93.


Matange N, Hunt DM, Buxton RS, Visweswariah SS.

Overexpression of the Rv0805 phosphodiesterase elicits a cAMP-independent transcriptional response.


Arshad N, Visweswariah SS.

Cyclic nucleotide signaling in intestinal epithelia: getting to the gut of the matter.


Nambi S, Gupta K, Bhattacharyya M, Ramakrishnan P, Ravikumar V, Siddiqui N, Thomas AT, Visweswariah SS.

Cyclic AMP-dependent protein lysine acylation in mycobacteria regulates fatty acid and propionate metabolism.

 J Biol Chem. 2013 May 17;288(20):14114-24.


Arshad N, Ballal S, Visweswariah SS.

Site-specific N-linked glycosylation of receptor guanylyl cyclase C regulates ligand binding, ligand-mediated activation and interaction with vesicular integral membrane protein 36 (VIP36)

J. Biol Chem. 2012  Feb 8;288(6):3907-17.


Arshad N, Visweswariah SS.

The multiple and enigmatic roles of guanylyl cyclase C in intestinal homeostasis.

FEBS Lett. 2012 Aug 31;586(18):2835-40.


Nambi S, BadiReddy S, Visweswariah SS, Anand GS.

Cyclic AMP-induced conformational changes in mycobacterial protein acetyltransferases.

J Biol Chem. 2012 May 25;287(22): 18115-29.


Fiskerstrand T, Arshad N, Haukanes, BI, Tronstad RR, Pham KD, Johansson S, Havik B, Tonder SL, Levy SE, BrackmanD, Boman H, Biswas KH, Apold J, Hovdenak N, Visweswariah SS, Knappskog PM.

Familial Diarrhea Syndrome caused by an activating GUCY2C activating mutation.

N. Engl. J. Med. 2012 Apr 26;366(17): 1586-95.


Dermol U, Janardan V, Tyagi R, Visweswariah SS, Podobnik M.

Unique utilization of a phosphoprotein phosphatase fold by a mammalian phosphodiesterase associated with WAGR syndrome.

J Mol Biol. 2011 Sep 23;412(3):481-94.


Chakraborti PK, Matange N, Nandicoori VK, Singh Y, Tyagi JS, Visweswariah SS.

Signalling mechanisms in mycobacteria.

Tuberculosis 2011 Sepl91(5):432-40.


Biswas KH, Visweswariah SS.

Distinct allostery Induced in the cyclic GMP-binding, cyclic GMP-specific phosphodiesterase (PDE5) by cyclic GMP, sildenafil and metal ions.

J. Biol Chem. 2011 Mar 11;286(10):8545-54.


Nambi S, Basu N, Visweswariah SS.

cAMP-regulated protein lysine acetylases in mycobacteria.

J. Biol Chem. 2010 Aug 6;285(32):24313-23.


Basu N, Arshad N, Visweswariah SS.

Receptor guanylyl cyclase (GC-C): regulation and signal transduction.

Mol. Cell Biochem. 2009 Jan. 334(1-2):7-80.


Podobnik M, Tyagi R, Matange N, Dermol U, Gupta AK, Mattoo R, Seshadri K, Visweswariah SS.

A mycobacterial cyclic AMP phosphodiesterase that moonlights as a modifier of cell wall permeability.

J. Biol. Chem. 2009 Nov 20;284(47):32846-57.


Saha S, Biswas, KH, Kondapalli C, Isloor N, Visweswariah SS.

The linker region in receptor guanylyl cyclases is a key regulatory module: mutational analysis of guanylyl cyclase C.

J. Biol. Chem. 2009 Oct2;284(40):27135-45.


Basu N, Bhandari R, Natarajan VT, Visweswariah SS.

Cross talk between receptor guanylyl cyclase C and c-src tyrosine kinase regulates colon cancer cell cytostasis.

Mol. Cell Biol. 2009 Oct;29(19):5277-89.


Sawarkar R, Visweswariah SS, Nellen W, Nanjundiah V.

Histone deacelylases regulate multicellular development in the social amoeba Dictyostelium discoideum.

J. Mol. Biol. 2009 Sep;391(5):833-48.


Biswas KH, Shenoy AR, Dutta A, Visweswariah SS.

The evolution of guanylyl cyclases as multidomain proteins: conserved features of kinase-cyclase domain fusions.

J. Mol. Evol. 2009 Jun;68(6):587-602.


Tyagi R, Shenoy AR, Visweswariah SS.

Characterization of an evolutionarily conserved metallophosphoesterase that is expressed in the fetal brain and associated with the WAGR syndrome.

J Biol Chem. 2009 Feb 20;284(8):5217-28.


Dass BK, Sharma R, Shenoy AR, Mattoo R, Visweswariah SS.

Cyclic AMP in mycobacteria: characterization and functional role of the Rv1647 ortholog in Mycobacterium smegmatis.

J Bacteriol. 2008 Jun;190(11):3824-34.


Biswas KH, Sopory S, Visweswariah SS.

The GAF domain of the cGMP-binding, cGMP-specific phosphodiesterase (PDE5) is a sensor and a sink for cGMP.

Biochemistry. 2008 Mar 18;47(11):3534-43.


Shenoy AR, Capuder M, Draskovic P, Lamba D, Visweswariah SS, Podobnik M.

Structural and biochemical analysis of the Rv0805 cyclic nucleotide phosphodiesterase from Mycobacterium tuberculosis.

J Mol Biol. 2007 Jan 5;365(1):211-25.


Shenoy AR, Visweswariah SS.

New messages from old messengers: cAMP and mycobacteria.

Trends Microbiol. 2006 Dec;14(12):543-50.


Shenoy AR, Visweswariah SS.

Mycobacterial adenylyl cyclases: biochemical diversity and structural plasticity.

FEBS Lett. 2006 Jun 12;580(14):3344-52.


Jaleel M, Saha S, Shenoy AR, Visweswariah SS.

The kinase homology domain of receptor guanylyl cyclase C: ATP binding and identification of an adenine nucleotide sensitive site.

Biochemistry. 2006 Feb 14;45(6):1888-98.


Ketkar AD, Shenoy AR, Ramagopal UA, Visweswariah SS, Suguna K.
A Structural Basis for the Role of Nucleotide Specifying Residues in Regulating the Oligomerization of the Rv1625c Adenylyl Cyclase from M.tuberculosis.
J Mol Biol. 2006 Mar 3;356(4):904-16.


Shenoy AR, Sreenath NP, Podobnik M, Kovacevic M, Visweswariah SS.
The Rv0805 gene from Mycobacterium tuberculosis encodes a 3’, 5’-cyclic nucleotide phosphodiesterase: biochemical and mutational analysis.
Biochemistry. 2005 Dec 6;44(48):15695-704.


Shenoy AR, Srinivas A, Mahalingam M, Visweswariah SS.
An adenylyl cyclase pseudogene in Mycobacterium tuberculosis has a functional ortholog in Mycobacterium avium.
Biochimie. 2005 Jun;87(6):557-63.

Shenoy AR, Sreenath NP, Mahalingam M, Visweswariah SS.
Characterization of phylogenetically distant members of the adenylate cyclase family from mycobacteria: Rv1647 from Mycobacterium tuberculosis and its orthologue ML1399 from M. leprae.
Biochem J. 2005 Apr 15;387(Pt 2):541-51.


Jaleel M, Shenoy AR, Visweswariah SS.
Tyrphostins are inhibitors of guanylyl and adenylyl cyclases.
Biochemistry. 2004 Jun 29;43(25):8247-55.


Sopory S, Kaur T, Visweswariah SS.
The cGMP-binding, cGMP-specific phosphodiesterase (PDE5): intestinal cell expression, regulation and role in fluid secretion.
Cell Signal. 2004 Jun;16(6):681-92.


Ghanekar Y, Chandrashaker A, Tatu U, Visweswariah SS.
Glycosylation of the receptor guanylyl cyclase C: role in ligand binding and catalytic activity.
Biochem J. 2004 May 1;379(Pt 3):653-63.


Shenoy AR, Visweswariah SS.
Class III nucleotide cyclases in bacteria and archaebacteria: lineage specific expansion of adenylyl cyclases and a dearth of guanylyl cyclases.
FEBS Lett. 2004;561:11-21.


Shenoy AR, Shivakumar K, Krupa A, Srinivasan N, Visweswariah SS.
A survey of nucleotide cyclases in Actinobacteria: unique domain organization and expansion of the class III cyclase family in Mycobacterium tuberculosis.
Comp. Fuct. Genomics 2004;5:17-38.


Ketkar AD, Shenoy AR, Kesavulu MM, Visweswariah SS and Suguna K.
Purification, crystallization and preliminary X-ray diffraction analysis of the catalytic domain of adenylyl cyclase Rv1625c from Mycobacterium tuberculosis.
Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):371-3.


Ghanekar Y, Chandrashaker A, Visweswariah SS.
Cellular refractoriness to the heat-stable enterotoxin peptide is associated with alterations in levels of the differentially glycosylated forms of guanylyl cyclase C.
Eur J Biochem. 2003 Sep;270(18):3848-57.
Shenoy AR, Visweswariah SS.
Site-directed mutagenesis using a single mutagenic oligonucleotide and DpnI digestion of template DNA.
Anal Biochem. 2003 Aug 15;319(2):335-6. No abstract available.
Thomas CJ, Sharma S, Kumar G, Visweswariah SS, Surolia A.
Biopanning of endotoxin-specific phage displayed peptides.
Biochem Biophys Res Commun. 2003 Jul 18;307(1):133-8.
Shenoy AR, Srinivasan N, Subramaniam M, Visweswariah SS.
Mutational analysis of the Mycobacterium tuberculosis Rv1625c adenylyl cyclase:  residues that confer nucleotide specificity contribute to dimerization.
FEBS Lett. 2003 Jun 19;545(2-3):253-9.
Sopory S, Balaji S, Srinivasan N, Visweswariah SS.
Modeling and mutational analysis of the GAF domain of the cGMP-binding, cGMP-specific phosphodiesterase, PDE5.
FEBS Lett. 2003 Mar 27;539(1-3):161-6.
Jaleel M, London RM, Eber SL, Forte LR, Visweswariah SS.
Expression of the receptor guanylyl cyclase C and its ligands in reproductive tissues of the rat: a potential role for a novel signaling pathway in the epididymis.
Biol Reprod. 2002 Dec;67(6):1975-80.
Manjunatha UH, Dalal M, Chatterji M, Radha DR, Visweswariah SS, Nagaraja V.
Functional characterisation of mycobacterial DNA gyrase: an efficient decatenase.
Nucleic Acids Res. 2002 May 15;30(10):2144-53.
Shenoy AR, Srinivasan N, Visweswariah SS.
The ascent of nucleotide cyclases: conservation and evolution of a theme.
J Biosci. 2002 Mar;27(2):85-91.
Ghosh YK, Visweswariah SS, Bhattacharya S.
Advantage of the ether linkage between the positive charge and the cholesteryl skeleton in cholesterol-based amphiphiles as vectors for gene delivery.
Bioconjug Chem. 2002 Mar-Apr;13(2):378-84.
Sindice A, Basoglu C, Cerci A, Hirsch JR, Potthast R, Kuhn M, Ghanekar Y, Visweswariah SS, Schlatter E.
Guanylin, uroguanylin, and heat-stable euterotoxin activate guanylate cyclase C and/or a pertussis toxin-sensitive G protein in human proximal tubule cells.
J Biol Chem. 2002 May 17;277(20):17758-64. Epub 2002 Mar 21.
Bhandari R, Srinivasan N, Mahaboobi M, Ghanekar Y, Suguna K, Visweswariah SS.
Functional inactivation of the human guanylyl cyclase C receptor: modeling and mutation of the protein kinase-like domain.
Biochemistry. 2001 Aug 7;40(31):9196-206.
Roy N, Guruprasad MR, Kondaiah P, Mann EA, Giannella RA, Visweswariah SS.
Protein kinase C regulates transcription of the human guanylate cyclase C gene.
Eur J Biochem. 2001 Apr;268(7):2160-71.
Manjunatha UH, Mahadevan S, Visweswariah SS, Nagaraja V.
Monoclonal antibodies to mycobacterial DNA gyrase A inhibit DNA supercoiling activity.
Eur J Biochem. 2001 Apr;268(7):2038-46.
Manjunatha UH, Somesh BP, Nagaraja V, Visweswariah SS.
A Mycobacterium smegmatis gyrase B specific monoclonal antibody reveals association of gyrase A and B subunits in the cell.
FEMS Microbiol Lett. 2001 Jan 1;194(1):87-92.

Vijayachandra K, Guruprasad M, Bhandari R, Manjunath UH, Somesh BP, Srinivasan N, Suguna K, Visweswariah SS.
Biochemical characterization of the intracellular domain of the human guanylyl cyclase C receptor provides evidence for a catalytically active homotrimer.
Biochemistry. 2000 Dec 26;39(51):16075-83.
Qian X, Prabhakar S, Nandi A, Visweswariah SS, Goy MF.
Expression of GC-C, a receptor-guanylate cyclase, and its endogenous ligands uroguanylin and guanylin along the rostrocaudal axis of the intestine.
Endocrinology. 2000 Sep;141(9):3210-24.
Ghosh YK, Visweswariah SS, Bhattacharya S.
Nature of linkage between the cationic headgroup and cholesteryl skeleton controls gene transfection efficiency.
FEBS Lett. 2000 May 19;473(3):341-4.

Bakre MM, Sopory S, Visweswariah SS.
Expression and regulation of the cGMP-binding, cGMP-specific phosphodiesterase (PDE5) in human colonic epithelial cells: role in the induction of cellular refractoriness to the heat-stable enterotoxin peptide.
J Cell Biochem. 2000 Feb;77(1):159-67.
Bakre MM, Ghanekar Y, Visweswariah SS.
Homologous desensitization of the human guanylate cyclase C receptor. Cell-specific regulation of catalytic activity.
Eur J Biochem. 2000 Jan;267(1):179-87.
Swaminathan CP, Nandi A, Visweswariah SS, Surolia A.
Thermodynamic analyses reveal role of water release in epitope recognition by a monoclonal antibody against the human guanylyl cyclase C receptor.
J Biol Chem. 1999 Oct 29;274(44):31272-8. Erratum in: J Biol Chem 2002 May 10;277(19):17374.
Bhandari R, Suguna K, Visweswariah SS.
Guanylyl cyclase C receptor: regulation of catalytic activity by ATP.
Biosci Rep. 1999 Jun;19(3):179-88.
London RM, Eber SL, Visweswariah SS, Krause WJ, Forte LR.
Structure and activity of OK-GC: a kidney receptor guanylate cyclase activated by guanylin peptides.
Am J Physiol. 1999 Jun;276(6 Pt 2):F882-91.
Pattanaik P, Sooryanarayana, Adiga PR, Visweswariah SS.
Refolding of native and recombinant chicken riboflavin carrier (or binding) protein: evidence for the formation of non-native intermediates during the generation of active protein.
Eur J Biochem. 1998 Dec 1;258(2):411-8.
Nandi A, Suguna K, Surolia A, Visweswariah SS.
Topological mimicry and epitope duplication in the guanylyl cyclase C receptor.
Protein Sci. 1998 Oct;7(10):2175-83.
Sooryanarayana, Sarkar S, Adiga PR, Visweswariah SS.
Identification and characterization of receptors for riboflavin carrier protein in the chicken oocyte. Role of the phosphopeptide in mediating receptor interaction.
Biochim Biophys Acta. 1998 Feb 17;1382(2):230-42.
Nandi A, Bhandari R, Visweswariah SS.
Epitope conservation and immunohistochemical localization of the guanylin/stable toxin peptide receptor, guanylyl cyclase C.
J Cell Biochem. 1997 Sep 15;66(4):500-11.
Bakre MM, Visweswariah SS.
Dual regulation of heat-stable enterotoxin-mediated cGMP accumulation in T84 cells by receptor desensitization and increased phosphodiesterase activity.
FEBS Lett. 1997 May 26;408(3):345-9.
Garrett BM, Visweswariah SS.
A conformational epitope in the N-terminus of the Escherichia coli heat-stable enterotoxins is involved in receptor-ligand interactions.
Biochim Biophys Acta. 1996 Nov 15;1317(2):149-54.
Nandi A, Mathew R, Visweswariah SS.
Expression of the extracellular domain of the human heat-stable enterotoxin receptor in Escherichia coli and generation of neutralizing antibodies.
Protein Expr Purif. 1996 Sep;8(2):151-9.
Sooryanarayana, Adiga PR, Visweswariah SS.
Hyperexpression of chicken riboflavin carrier protein: antibodies to the recombinant protein curtail pregnancy in rodents.
Protein Expr Purif. 1996 Mar;7(2):147-54.
Visweswariah SS, Ramachandran V, Ramamohan S, Das G, Ramachandran J.
Characterization and partial purification of the human receptor for the heat-stable enterotoxin.
Eur J Biochem. 1994 Feb 1;219(3):727-36.
Visweswariah SS, Shanthi G, Balganesh TS.
Interaction of heat-stable enterotoxins with human colonic (T84) cells: modulation of the activation of guanylyl cyclase.
Microb Pathog. 1992 Mar;12(3):209-18.
Dwarakanath P, Visweswariah SS, Subrahmanyam YV, Shanthi G, Jagannatha HM, Balganesh TS.
Cloning and hyperexpression of a gene encoding the heat-stable toxin of Escherichia coli.
Gene. 1989 Sep 30;81(2):219-26.
Visweswariah SS, Adiga PR.
Estrogen modulation of riboflavin carrier protein in the bonnet monkey (Macaca radiata).
J Steroid Biochem. 1988 Jul;31(1):91-6.
Visweswariah SS, Adiga PR.
Purification of a circulatory riboflavin carrier protein from pregnant bonnet monkey (M. radiata): comparison with chicken egg vitamin carrier.
Biochim Biophys Acta. 1987 Sep 24;915(2):141-8.
Visweswariah SS, Karande AA, Adiga PR.
Immunological characterization of riboflavin carrier proteins using monoclonal antibodies.
Mol Immunol. 1987 Sep;24(9):969-74.
Visweswariah SS, Adiga PR.
Isolation of riboflavin carrier proteins from pregnant human and umbilical cord serum: similarities with chicken egg riboflavin carrier protein.
Biosci Rep. 1987 Jul;7(7):563-71.
Karande AA, Visweswariah SS, Adiga PR.
A rapid method of epitope analysis using Superose 12 gel filtration. A study with monoclonal antibodies to chicken riboflavin carrier protein.
J Immunol Methods. 1987 May 20;99(2):173-7.


Home Research Activities Lab Members Publications Collaborators


Indian Institute of Science • Dept. of MRDG